Part:BBa_K4488021:Design

Fusion of free-use BFP with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 151
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

One consideration was the addition of the flexible linker in order to optimise protein folding.

Source

Original GFP is derived from the jellyfish Aequorea victoria. Free use GFP was designed by Mark Somerville (see part BBa_K3814004)